A novel model for the investigation of orthotopically growing primary and secondary bone tumours using intravital microscopy

Article de journal


Auteurs / Editeurs


Domaines de Recherche


Détails sur la publication

Liste des auteurs: Sckell A
Editeur: SAGE Publications (UK and US)
Année de publication: 2005
Numéro du volume: 39
Numéro de publication: 4
Page d'accueil: 377
Dernière page: 383
Nombre de pages: 7
ISSN: 0023-6772
Languages: Anglais-Royaume-Uni (EN-GB)


Résumé

Here is reported the development of an experimental model using
intravital microscopy as a tool to orthotopically investigate malignant
bone tumours. Although up to 85% of the most frequently occurring
malignant solid tumours, such as lung and prostate carcinomas,
metastasize into the bone, and despite the knowledge that a tumour's
course may be altered by its surrounding tissue, there is no adequate
experimental model available enabling the investigation of
orthotopically grown bone tumours 112 vivo. Intravital microscopy is an
internationally accepted experimental method, used in various acute and
chronic animal models, that enables qualitative and quantitative
analysis of the angiogenesis, microcirculation, growth behaviour, etc.
of various benign and malignant tissues. Non-invasive investigations of
up to several weeks are possible. Additionally, tissue samples can be
taken after termination of the in vivo experiments for further ex vivo
investigation (histology, immunohistochemistry, molecular biology,
etc.), elucidating the mechanisms that underlie the in vivo
observations. Severe combined immunodeficient mice were fitted with a
cranial window preparation where the calvaria served as the site for
orthotopic implantation of the solid human tumours Saos-2 osteosarcoma
(primary) and A 549 lung carcinoma and PC-3 prostate carcinoma
(secondary). In all preparations, the take rate was 100%. Histological
assessment confirmed the data obtained in vivo, showing typical tumour
growth with infiltration of the surrounding osseous and soft tissues.
This novel model serves as a valuable tool in understanding the biology
of primary and secondary bone tumours in physiological and
pathophysiological situations, with implications for the most areas of
tumour therapy such as chemotherapy, radiation and antiangiogenesis. ©
Laboratory Animals Ltd.


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