Dosage of inhaled nitric oxide

Journal article


Research Areas

Publication Details

Subtitle: A simple method for experimental studies
Author list: Sckell A
Publisher: Springer Verlag (Germany)
Publication year: 1999
Volume number: 199
Issue number: 3
Start page: 121
End page: 128
Number of pages: 8
ISSN: 0300-9130
Languages: English-Great Britain (EN-GB)


Few studies on treatment with inhaled nitric oxide (NOi) have been
carried out in small laboratory animals yet, since commercially
available dosing devices are not appropriate in this setting for
technical or financial reasons. The aim of our study was to establish
and validate a simple, cost-effective system for the application of NOi
in small animals. The system mixes NOi with constant-flow inspiratory
gas. A gas blender allows for a mixture of nitrogen, oxygen, and NO
dissolved in nitrogen. A formula using the desired inspiratory oxygen
fraction and the desired concentration of NOi as independent variables
derives a somewhat higher inspiratory oxygen fraction, which is preset
using an oximeter. Then the flow of NO in nitrogen is started, lowering
the inspiratory oxygen fraction to the initially desired value, thereby
adding NOi in the desired concentration. The method was validated by 153
adjustments, covering a variety of oxygen fractions and concentrations
of NOi. NOi was measured by chemiluminescence as reference method. A
close correlation (R = 0.994) was found, and the regression line was
close to the line of identity with y = -0.0994 + 1.048x. No systematic
errors could be identified. We conclude that the method described may
serve as a simple, cost-effective way to administer NOi to small


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