Quantitative assessment of angiogenesis and osteogenesis after transplantation of bone. Comparison of isograft and allograft bone in mice

Journal article


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Publication Details

Author list: Sckell A
Publication year: 1999
Volume number: 70
Issue number: 4
Start page: 374
End page: 380
Number of pages: 7
Languages: English-Great Britain (EN-GB)


We performed a vital microscopic study in mice bearing dorsal skinfold
chambers to characterize microvascular perfusion and
leukocyte/endothelium interaction and their effects on elongation and
mineralization of neonatal isograft and allograft bone. Isograft (C57/BL
to C57/BL) and allograft bone (C57/BL to BALB/C) revascularized
simultaneously. However, vascular perfusion and density were lower in
allograft bone than in isograft bone. Leukocyte/endothelium interaction
was the same in isograft and allograft bones. Revascularization was not
detected in allograft bone transplanted to presensitized recipients.
Moreover, in preexisting vessels at the transplantation site,
leukocyte/endothelium interaction was altered in allograft bone of
presensitized recipients, despite a normal systemic leukocyte count.
Femoral growth resulting from thickening of both epiphyses did not
differ between experimental groups, however, mineralization occurred in
isograft bone only. Isograft bone was histologically intact, allograft
bone hypovital and allograft bone in presensitized recipients necrotic
12 days after implantation. Our findings suggest that graft
incorporation or rejection is mediated by the microvasculature and that
presensitizing of recipients accelerates rejection of allograft bone.


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Last updated on 2018-18-07 at 17:50