Highly sensitive peptide-based probes for protein tyrosine phosphatase activity utilizing a fluorogenic mimic of phosphotyrosine

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Author list: Mitra S, Barrios AM
Publisher: Elsevier
Publication year: 2005
Volume number: 15
Issue number: 23
Start page: 5142
End page: 5145
Number of pages: 4
ISSN: 0960-894X
Languages: English-Great Britain (EN-GB)


Abstract

Fluorescent probes that can be incorporated into peptides and proteins are in high demand, with applications ranging from cellular imaging to binding and activity assays. Here, we report the high yielding synthesis of an enantiomerically pure phosphocoumarin-based amino acid and its incorporation into peptides via standard solid-phase peptide synthesis methodologies. Peptides containing this new amino acid serve as highly sensitive fluorogenic probes for protein tyrosine phosphatase activity. (c) 2005 Elsevier Ltd. All rights reserved.


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Last updated on 2019-23-08 at 11:15