Improved Production of Bacillus thuringiensis by Intermittent Fed-Batch Culture with Total Cell Retention

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Publication Details

Author list: Habeych E, Barrios AFG, Restrepo-Boland A, Orduz S
Publisher: De Gruyter
Publication year: 2010
Journal: International Journal of Chemical Reactor Engineering (1542-6580)
Volume number: 8
Number of pages: 8
ISSN: 1542-6580
Languages: English-Great Britain (EN-GB)


Abstract

Intermittent fed-batch culture with total cell retention was evaluated during Bacillus thuringiensis serovar. kurstaki fermentations aiming to enhance biomass and spore concentration yields while maintaining toxicity. This method is derived from the already reported intermittent fed-batch culture and utilized a tangential flow filtration system for cell retention. After 4 pulses, we obtained a biomass of 74.36 g.L-1 and this biomass displayed a half lethal concentration towards neonate Spodoptera frugiperda larvae of 40.53 ng.cm(-2), and spore concentration of 4.6 x 1014 CFU mL(-1). On the other hand, volumetric productivity was increased seven-fold compared to batch fermentation. Interestingly, we are not aware of higher spore concentrations reported to date for B. thuringiensis cultures.


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Last updated on 2019-23-08 at 11:15