Phage Phi Pan70, a Putative Temperate Phage, Controls Pseudomonas aeruginosa in Planktonic, Biofilm and Burn Mouse Model Assays

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Author list: Holguin AV, Rangel G, Clavijo V, Prada C, Mantilla M, Gomez MC, Kutter E, Taylor C, Fineran PC, Barrios AFG, Vives MJ
Publisher: MDPI
Publication year: 2015
Volume number: 7
Issue number: 8
Start page: 4602
End page: 4623
Number of pages: 22
ISSN: 1999-4915
Languages: English-Great Britain (EN-GB)


Abstract

Pseudomonas aeruginosa is one of the Multi-Drug-Resistant organisms most frequently isolated worldwide and, because of a shortage of new antibiotics, bacteriophages are considered an alternative for its treatment. Previously, P. aeruginosa phages were isolated and best candidates were chosen based on their ability to form clear plaques and their host range. This work aimed to characterize one of those phages, phi Pan70, preliminarily identified as a good candidate for phage-therapy. We performed infection curves, biofilm removal assays, transmission-electron-microscopy, pulsed-field-gel-electrophoresis, and studied the in vivo phi Pan70 biological activity in the burned mouse model. phi Pan70 was classified as a member of the Myoviridae family and, in both planktonic cells and biofilms, was responsible for a significant reduction in the bacterial population. The burned mouse model showed an animal survival between 80% and 100%, significantly different from the control animals (0%). However, analysis of the phi Pan70 genome revealed that it was 64% identical to F10, a temperate P. aeruginosa phage. Gene annotation indicated phi Pan70 as a new, but possible temperate phage, therefore not ideal for phage-therapy. Based on this, we recommend genome sequence analysis as an early step to select candidate phages for potential application in phage-therapy, before entering into a more intensive characterization.


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Last updated on 2019-23-08 at 11:15